As it is a recurring task for QBDC to help in the (re)design of a biopharmaceutical production facility, we have developed an in-depth cost calculation sheet, which may serve as a scratchboard for facility planning and repurposing projects. It focuses on single-use, i.e. disposable technologies, facilities, but may as well be used for stainless steel facilities. The calculation include machines, utilities, consumables, personnel amongst others and may be adjusted and modified easily if needed.